Analytical Development, Characterization and Validation of Cost Effective Stability Indicating High Performance Liquid

Abstract

Simple and sensitive stability indicating HPLC method was developed for the assurance of Carisoprodol in mass and in their tablet formulation. Effective chromatographic separation was accomplished on Zorbax Eclipse in addition to C18 (250 x 4.6 mm; 5 ?m molecule size) analytical segment through isocratic elution mode. The versatile stage made out of 10mM potassium dihydrogen orthophosphate-methanol-acetonitrile in the proportion of 60:20:20 (v/v/v). Detection was performed at 240 nm. Analytical execution of the proposed method was statistically validated regarding linearity, exactness, precision, heartiness, roughness, explicitness, detection and evaluation limits. The linearity range was 1-30 ?g/ml with relationship coefficient 0.9995. The debasement items acquired were all around settled from the carisoprodol. The validated stability indicating HPLC method was effectively connected to the examination of Carisoprodol in their pharmaceutical tablets. The procedure was measured linearly in the range of 2.5–17.5 ?g / mL for PYR, 25–175 ?g / mL for ETH, and 40–280 ?g / mL for MOX with 0.125 ?g / mL/1.28 ?g / mL, 0.25 ?g / mL/2.56 ?g / mL, and 0.35 ?g / mL/3.65 ?g / mL, respectively. Oxidative, hydrolytic, photolytic, and warm oxidation were also identified with the medications; the defilement items showed impedance with measurement of PYR, ETH, and MOX. The suggested approach was expected to include quantitative tablet descriptions of MOX (400 mg), ETH (250 mg) and PYR (25 mg) in fixed bit combination.