In Vitro Germination, Callus Induction and Shoot Multiplication of Important Medicinal Plant, Christia Vespertilionis (L.F.) Bakh.F.
Christia vespertilionis (L. f.) Bakh. f. is an important medicinal plant belongs to Fabaceae family that plant commonly known as butterfly wing or ‘rerama’. It has been used in traditional medicine and was reported to have anticancer, anti-inflammation and antiplasmodium activity. In vitro culture technique potentially can enhance the propagation and bioactive compounds production from this unexploited medicinal plant. This study established the in vitro cultures of C. vespertilionis using seed explants and aseptic seedlings. Four different pre-treatments were used to induce the in vitro germination consist of 50% (v/v) HCl, 0.2% (w/v) KNO3, 0.3 g/L GA3 and distilled water as control. Seeds treated with 50% (v/v) HCl showed the highest percentage of germination. Induction of callus and shoot multiplication was initiated from aseptic seedlings in MS medium supplemented with different concentration of 6-Benzylaminopurine (BAP) in combination with 1-Naphthaleneacetic acid (NAA) or 2,4-Dichlorophenoxyacetic acid (2,4-D). Explants of leaf, petiole and stem were produced different colour of callus consist of green, brownish green and yellowish green. The highest callus and multiple shoot production were observed in stem explant cultured on MS medium augmented with 2.0 mg/L BAP and 1.0 mg/L 2,4-D. Green callus was only produced in medium supplemented with NAA whereas green and brownish green callus was observed in medium supplemented with 2,4-D. Compact callus was produced in MS medium with combination of 1.0 mg/L BAP and 1.0 mg/L NAA while most friable callus were produced in MS medium supplemented with 2,4-D. This study is the first to report on the production of callus and multiple shoots from aseptic seedlings of C. vespertilionis.
Keywords:Callus, Christia vespertilionis, 2,4-dichlorophenoxyaceticacid.,seed germination, shoots multiplication.