Penetration depth of AH- plus Sealer in Dentinal Tubules following ErCr: YSGG Laser or EDTA application: A Confocal Laser Microscopic Study

Abstract

The aim of this study was to investigate the AHplus sealer penetration into dentinal tubule following smear layer removal by either use of ErCr:YSGG laser or EDTA. Thirty extracted human teeth with straight single roots were used. the teeth were decrownated, and the root canals were instrumented with ProTaper Next rotary instruments(DentsplyMaillefer, Ballagues, Switzerland) with copious irrigatant 2 mL 5% NaOCl. Smear layer removal was done for 20 of teeth either with ErCr:YSGG laser or with 17% EDTA, the remaining teeth used as controll. The canals were dried and obturated by using rhodamine B–labeledAHplus in combination with the carrier-based technique or the cold lateral compaction. After setting, the roots were sectioned horizontally at 3, 6, and 9-mm distances from the apical tip. On each section, sealer penetration in the dentinal tubules was measured by using confocal laser scanning microscopy. When groups were compared statistically, significant differences were found between laser/carrie-based group and all group (at P ?. 01),and between laser/lateral compaction, EDTA/carrier-based groups and  other groups. i it is obvious that using of ErCr: YSGG laser for smear layer removal with the carrier-baseed technique offers greater dentinal tubule penetration of the sealers.