A Study on Production and Evaluation of L-Asparaginase Obtained From BACILLUS SUBTILIS

Authors

  • Senthil Kumar Jeyaraj
  • Baby Shakila Parimelazhagan
  • Vishnu Priya Kalimuthu
  • Varshini Sivaram
  • Agalya Perumal

Abstract

L-asparaginase is commonly referred as one of the most acceptable clinical anti-tumor agents against lymphosarcoma and its associated leukemia. L-asparaginase (L-asparaginase amino hydrolase) mediates the catalysis through hydrolysis of L-asparagines into aspartic acid and ammonia. L-asparaginase was isolated from bacteria (Bacillus subtilis) by submerged fermentation process. The productions of this enzyme were subjected to physical parameters like pH, temperatures and different days. The outcome of this research indicated the pH 8, 300 C, and days 3 were best suited and optimal environmental condition for Bacillus subtilis. The enzyme was partially purified using the acetone method and stored to proceed further.

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Published

2020-01-22

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Articles