Protein-Protein Interaction for Neuraminidase Enzyme using Graph Theory Software (Cytoscape)

Abstract

There are seven biological classifications in influenza virus. Out of 7 only four types of influenza viruses cause the influenza fever in human, birds and mammals. Hemagglutinin [HA] and neuraminidase [NA] are the enzyme proteins available around the influenza virus A. Viral neuraminidase divided the sialic acid residues from the glycoprotein.  Hemagglutinin replicates the influenza virus A. Structural information of 376 Neuraminidase enzyme proteins and 162 Hemagglutinin proteins are available in PDB database. Proteins are binds with one protein to many proteins. Interactions between proteins are very much useful for drug design research. Few of the software are available for protein-protein interaction research. In this paper we are using freely available IntAct database for bimolecular interaction like protein-protein interaction analysis. We are also using network analysis software Cytoscape for protein-protein interaction study. Using IntAct database we were obtained 575 interaction for neuraminidase protein database. We have submitted the viral neuraminidase data from IntAct to the network analysis software Cytoscape. From the 575 protein-protein interaction for neuraminidase enzyme, we have found most of the proteins have one to two interactions. But two of the neuraminidase enzymes proteins P03468 and P03452 have 20 and 35 protein-protein interactions respectively. Above two neuraminidase proteins have play an important role for the drug design in influenza virus A. Graph theory analysis also carried for 575 protein interaction.