Detection of Blandm-1 Gene Encoding for MBL Resistance among Clinical Isolates of Pseudomonas Aeruginosa
Abstract
New Delhi metallo beta lactamase-1 ( NDM-1), is a newly identified class B ?-lactamase gene and found worldwide. BlaNDM-1 producing isolates always shows multidrug resistant patterns, as they possess various genes that encode for resistance to various antibiotics. In this study we aim to elucidate the antibiotic susceptibility pattern of 20 clinical isolates of P. aeruginosa and followed by the detection of blaNDM-1 gene by PCR. We found multiple antibiotic resistance even to carbapenem antibiotics. We found only one isolate was confirmed to possess this gene by PCR, this warrants the necessary to screen for this gene and to follow the antibiotic policies. There are only 2 reports of the presence of this gene in patients from Serbia. Though the reports are very limited, knowledge of its prevalence is very important as pseudomonas aeruginosa is purely a nosocomial pathogen with stubborn colonization capability and ability to persist in different environments of clinical setup. Pseudomonas aeruginosa isolates were detected for the presence of blaNDM-1 gene by PCR analysis. Detection of the gene was carried out using primer.
